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To become the cytoplasm (Table 1). The person PSORT II algorithms revealed
Apparently, AMID and endoG usually do not colocalize sig-Page 3 of(web page number not for citation PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28914615 Phosphorylcholine chemical information purposes)Journal of Biomedical Science 2009, 16:http://www.jbiomedsci.com/content/16/1/Figure 1 Sequence analysis and prediction of cellular areas of proteins Sequence evaluation and prediction of cellular locations of proteins. (A) Homology of sequences of AIF and AMID with detected conserved domains. (B) Sequences of AIF, AMID, and endoG with detected conserved domains and predicted web pages. Pyr_Redox ?NADH binding domain inside a larger FAD binding domain of pyridine nucleotide-disulphide oxidoreductase; Ndh ?FAD-containing subunit of NADH dehydrogenase; Nuc ?DNA/RNA non-specific endonuclease; MLS ?mitochondrial localization sequence; NLS ?nuclear localization signal; M ?N-myristoylation-allowing motif; TM ?transmembrane region.Web page four of(web page quantity not for citation purposes)Journal of Biomedical Science 2009, 16:http://www.jbiomedsci.com/content/16/1/Table 1: Prediction of cellular localization.Endonuclease G mitochondrion cytoplasm endoplasmic reticulum Golgi apparatus lysosome/vacuole peroxisome plasma membrane nucleus cystoskeleton AIF mitochondrion cytoplasm endoplasmic reticulum Golgi apparatus lysosome/vacuole peroxisome plasma membrane nucleus cystoskeleton AMID mitochondrion PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26740125 cytoplasm endoplasmic reticulum Golgi apparatus lysosome/vacuole peroxisome plasma membrane nucleus cystoskeleton Cyclophilin A mitochondrion cytoplasm endoplasmic reticulum Golgi apparatus lysosome/vacuole peroxisome plasma membrane nucleus cytoskeleton HSP70-1 mitochondrion cytoplasm endoplasmic reticulum Golgi apparatus lysosome/vacuole peroxisome plasma membrane nucleus cytoskeletonPSORT II ( ) 30.4 34.8 eight.7 8.7 8.7 8.7 -WoLF PSORT ( ) 25.9 six.9 ten.3 13.eight 15.five three.4 -MultiLoc ( ) 98.0 0.0 1.0 -Cello (reliability) 3.08 0.33 0.To be the cytoplasm (Table 1). The person PSORT II algorithms revealed the MLS in endoG sequence to be the N-terminal 48-amino-acid cleavable signal peptide [46,47] and the nuclear localization signal (NLS) at position 24 [48] inside the predicted MLS (Fig. 1B). In the sequence of AIF isoform 1 we predicted the MLS to be the N-terminal 61-amino-acid cleavable signal peptide [46,47], the NLS at positions 106 ?112 [48], and a single transmembrane segment between positions 68 ?84 (Fig. 1B) [27]. The PSORT II algorithms found an N-myristoylation-allowing motif [49] which would potentially permit incorporation of AMID into different cellular membranes [50] and 1 transmembrane segment between positions 11 ?33 by TMHMM 2.0 server (Fig. 1B) [51]. Nmyristoylation-allowing motif was also detected by two other bioinformatic tools ?the Myristoylator [52] and also the NMT Predictor [53], which defined the motif to be the amino acid sequence GSQVSVESGALHVVIVG starting at position two. In the sequence of HSP70-1 were detected NLS at positions 246 ?273 and 594 ?597. PSORT II and TMHMM two.0 identified nothing of interest for cyclophilin A. Within the sequence of DNA topoisomerase II have been predicted a number of NLS involving amino acids 632 to 1468. Experimental cellular locations of proteins Experimental determination of your cellular areas of studied proteins was performed either by transfecting living cells with mammalian expression vectors encoding the fusion proteins or by immunostaining of fixed cells by fluorescently labeled main antibody. Figure 2A shows a typical signal distributions of endoG-EYFP and AMIDtHcRed fluorescence in transfected human living U-2 OS cells.
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